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KMID : 1035320170440040291
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Korean Society for Dental Materials
2017 Volume.44 No. 4 p.291 ~ p.298
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Effects of conditioned media from phosphatidylserine-liposome-treated macrophages on the differentiation of human dental pulp cells
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Park Hee-Chul
Quan Hongxuan
Kim Yong-Joon
Yang Hyeong-Cheol
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Abstract
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This study aimed to examine the effect of phosphatidylserine-treated macrophages (THP-1) on the differentiation of human dental pulp cells. Phosphatidylserine, phosphatidylcholine, and cholesterol were mixed at a molar ratio of 2:1:1, and liposomes were produced via the vacuum evaporation method. Conditioned media (CM) were collected after treating macrophages, with liposomes for 6 and 24h, and were used for differentiating human dental pulp cells. To confirm the differentiation, we performed an evaluation of alkaline phosphatase (ALP) activity, reverse transcriptase polymerase chain reaction (RT-PCR) of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) gene expressions, and extracellular matrix mineralization assay via alizarin red S staining. There was a statistically significant increase in alkaline phosphatase activity with the conditioned medium containing 10% in the overall culture medium. RT-PCR assay revealed that conditioned media did not increase the mRNA expressions of DSPP and OCN genes. The degree of matrix mineralization was not affected by the conditioned medium. These results confirm a slight effect of the conditioned medium on the differentiation of human dental pulp cells. Future studies may adopt a method in which macrophages and human dental pulp cells are closely co-existed, to evaluate the effect of phosphatidylserine on the interaction between these cell types.
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KEYWORD
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Differentiation, Human dental pulp cell, Macrophage, Phosphatidylserine
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